Co-ordinated combination of Embden-Meyerhof-Parnas pathway and pentose phosphate pathway in Escherichia coli to promote L-tryptophan production

In this study, phosphoenolpyruvate and erythrose-4-phosphate are efficiently supplied by collaborative design of Embden-Meyerhof-Parnas (EMP) pathway pentose phosphate (PP) in Escherichia coli, thus increasing the L-tryptophan production. Firstly, effects disrupting EMP on production were studied, results indicated that strain with deletion phosphofructokinase A (i.e., E. coli JW-5 ΔpfkA) produced 23.4 ± 2.1 g/L However, glucosephosphate isomerase is not conducive to glucose consumption cell growth, especially isomerase. Next, carbon flux PP was enhanced introduction desensitized glucose-6-phosphate dehydrogenase (zwf) 6-phosphogluconate (gnd) 26.5 3.2 vs. 21.7 1.3 g/L) without obviously changing growth 0.41 h−1 0.44 h−1) as compared original JW-5. Finally, co-modifying investigated. It found well zwf gnd zwf243 gnd361 31.9 2.7 L-tryptophan, which 47.0% higher than addition, rate ΔpfkA increased despite bad The study have important reference value for following application metabolic engineering improve aromatic amino acids producing strains.